I am using constant systems French press. There is lot of volume loss and frothing happening. I am working with bacterial cell suspension and volumes are around 1-2 ml. All kind of suggestions are welcome.
Do not leave a space inside the cell. Before you close the cell, push the piston to the liquid level. I am assuming that you use a smaller french press.
I am using the one shot model
http://www.constantsystems.com/products/oneshot.php
At this volume, why don't you use a sonicator to disrupt the cells? it's very effective and simple
I agree with Loc Trinh. But for some membrane protein sonication is not good.
There is a new device called the PCT Shredder Driver. It seems designed for small volumes and claim that works fine. You may get more detailed in formation at www.pressurebiosciences.com/products/the-pct-shredder.html
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