Harinder Singh

11 Questions 34 Answers 0 Followers

Questions related from Harinder Singh

How much 100mM of Gluconic acid can change pH of a buffer?

1. 100mM of glucose is getting catalyzed in solution and end product is gluconate. Theoretically, how much pH change will happen if reaction is occurring in 100mM buffer of pH7. 2. If I want to...

12 December 2014 3,362 1 View

What are the most suitable conditions for E. coli DE3 sonication?

I am sonicating DE3 cells for extracting my recombinant protein. I am getting my protein and things are working fine. But, I am curious to know about over or under sonication and overestimation...

09 September 2014 351 4 View

How can one decide on DNA amount, plating amount and screening size in mutant library construction?

During mutant library (e.g. 1 kb gene product, protein mutant library) preparation: 1. How much of DNA (ng/ug of constructed library) is transformed? 2. How much of a transformation mix...

09 September 2014 5,887 4 View

What is the reason for my huge solvent peak?

I am running my GC samples in ethyl acetate solvent. FID/Injector-220 degree. Sample volume 2 ul. I tried different ramping, but I am getting huge peak (solvent) in the beginning every time. What...

09 September 2014 2,722 19 View

Anybody using Dynabeads?

I want to use oligoDT Dynabeads for mRNA isolation. Do you need any specific magnetic platform. Can I use the magnetic stirrer as magnet source for bead separation.

01 January 2013 3,804 4 View

What is the trick to working with low volumes in a french press?

I am using constant systems French press. There is lot of volume loss and frothing happening. I am working with bacterial cell suspension and volumes are around 1-2 ml. All kind of suggestions are...

08 August 2012 9,597 5 View

What is the most important feature/parameter for considering a gene as a pseudogene?

If its reading frame has a start codon and no stop codons in between, is there any chance that it will make a protein?

08 August 2012 9,404 6 View

Using western blot apparatus for northern blotting?

My lab has a regular set up for protein electrophoresis and blotting. I want to use the same for RNA blotting. Which recipe is the best and least time consuming to make my whole setup RNase free?...

06 June 2012 2,236 4 View

Rule out the possibility of tRNA/rRNA in smallRNA population?

I have some smallRNA candidates. How can I confirm that they are tRNA/rRNA, any online software/program/tool available?

06 June 2012 6,003 2 View

What methods are there other than northern blotting for small RNA validation in prokaryotes?

I have some intergenic small RNA candidates in prokaryotes which I want to validate.

06 June 2012 2,651 7 View

Is there any tool for analyzing intergenic regions?

In order to filter out sequences on the basis of size, and stop codons.

06 June 2012 6,817 0 View