In our laboratory, while isolating disease-causing fungal phytopathogens; we faced immense troubles due to contamination. Bacterial contamination were inhibited by using various antibacterial such as Kanamycin monosulfate, Ampicillin trihydrate, Gentamicin, Chloramphenicol etc. However fungal contamination is still a major obstacle.
Will cycloheximide usage will come in handy?
Phil Geis I am trying to isolate the pathogenic fungi responsible for the disease, the saprophytic/ opportunistic moulds are contaminating the isolating cultures
Phil Geis I do not know!
That is why I have asked for experienced help on whether using cycloheximide will be effective or not!
I have successfully isolated my target fungi but it took very long time due to high contamination rate. That is why I am looking for some guidance here Dear Sir.
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