Anyone used Presenilin 1 [C1C2] Antibody by GeneTex?

Hello, I'm performing WB analysis using the Antibody anti-Presenilin 1 [C1C2] (cat number GTX116016) of the GeneTex company. The datasheet specifies that this antibody recognizes the full-length...

03 August 2020 7,086 1 View

How to treat mammalian diel activity patterns as a continuous variable?

Does anyone have experience in treating/converting mammalian diel activity patterns (diurnal/nocturnal/crepuscular/cathemeral) as a continuous variable rather than a factorial one? I am thinking...

13 May 2020 9,562 2 View

Any suggestions for mouse anti-phospho-ERK1/2 antibodies?

Hello, I am looking for a mouse anti-phospho ERK1/2 to perform immunofluorescence staining on cells (human iPSCs). I have experience with rabbit anti-phospho ERK1/2 #4370 from Cell Signalling...

24 April 2019 8,070 4 View

What's the dosage of Dexmedetomidine that you are using for the Drug Induced Sleep Endoscopy?

18 November 2018 1,703 1 View

DNA Output using QIAGEN Plasmid Miniprep kit?

Hi, I used for the first time the QIAGEN Plasmid Miniprep kit to extract DNA from my plasmids. After DNA extraction I've quantified the DNA concentration and the result is a DNA around 200ng/ul...

01 August 2018 5,123 6 View

What is the simplest and cheapest method for coating glass with a thin 100 micron uniform layer of epoxy or silicone resin?

I want to make a uniform thin coat of resin /pigment on 5*100 cm glass sheets. The glass could be cut before or after the coating is applied. The coating should be very even in thickness. Any...

10 February 2018 2,569 5 View

Why by CRIPR-Cas9 I get only point mutations and how can I induce disruption of my protein of interest?

I performed CRISPR/Cas9 in CHO cells to KO the protein Arrestin3. I transfected cells with a single plasmid containing gRNA - Cas9 - YFP. Then I sorted for YFP and I screened clones by sequencing....

26 January 2017 8,704 2 View

Any suggestions on Alcian blue/nuclear fast red counterstain on paraffin slides?

Hi, I'm going to try an alcian blue/nuclear fast red staining on heart mouse paraffin slides. All the protocols that I've found in internet provide a 30' of incubation in alcian blue. Is this time...

23 April 2015 9,149 9 View

How can I measure the concentration of C60 fullerene (nanoparticles) in tissues?

I have exposed clams in vivo to C60 fullerene for 7 days. And I would like to measure a possible accumulation in gills and digestive gland. If you can help me. Thanks in advance.

17 December 2014 5,369 6 View

Could a transfected promoter be susceptible to epigenetic modifications?

I am trying to understand if the promoter that I want to stably transfect into the cells through the use of a plasmid could be epigenetically modified, as occurred for the endogenous promoter. Thanks.

26 October 2014 3,353 6 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View