Anyone used Presenilin 1 [C1C2] Antibody by GeneTex?

Hello, I'm performing WB analysis using the Antibody anti-Presenilin 1 [C1C2] (cat number GTX116016) of the GeneTex company. The datasheet specifies that this antibody recognizes the full-length...

03 August 2020 7,086 1 View

How to treat mammalian diel activity patterns as a continuous variable?

Does anyone have experience in treating/converting mammalian diel activity patterns (diurnal/nocturnal/crepuscular/cathemeral) as a continuous variable rather than a factorial one? I am thinking...

13 May 2020 9,562 2 View

Any suggestions for mouse anti-phospho-ERK1/2 antibodies?

Hello, I am looking for a mouse anti-phospho ERK1/2 to perform immunofluorescence staining on cells (human iPSCs). I have experience with rabbit anti-phospho ERK1/2 #4370 from Cell Signalling...

24 April 2019 8,070 4 View

What's the dosage of Dexmedetomidine that you are using for the Drug Induced Sleep Endoscopy?

18 November 2018 1,703 1 View

DNA Output using QIAGEN Plasmid Miniprep kit?

Hi, I used for the first time the QIAGEN Plasmid Miniprep kit to extract DNA from my plasmids. After DNA extraction I've quantified the DNA concentration and the result is a DNA around 200ng/ul...

01 August 2018 5,123 6 View

RNA integrity number for urine RNA?

Dear colleagues, Do you know the minimum RNA integrity number (RNI) accepted for urine RNA in order to consider the sample for the final analysis? Any reference? Thanks, Ilaria

04 November 2017 1,320 2 View

Why by CRIPR-Cas9 I get only point mutations and how can I induce disruption of my protein of interest?

I performed CRISPR/Cas9 in CHO cells to KO the protein Arrestin3. I transfected cells with a single plasmid containing gRNA - Cas9 - YFP. Then I sorted for YFP and I screened clones by sequencing....

26 January 2017 8,704 2 View

Any suggestions on Alcian blue/nuclear fast red counterstain on paraffin slides?

Hi, I'm going to try an alcian blue/nuclear fast red staining on heart mouse paraffin slides. All the protocols that I've found in internet provide a 30' of incubation in alcian blue. Is this time...

23 April 2015 9,149 9 View

How can I measure the concentration of C60 fullerene (nanoparticles) in tissues?

I have exposed clams in vivo to C60 fullerene for 7 days. And I would like to measure a possible accumulation in gills and digestive gland. If you can help me. Thanks in advance.

17 December 2014 5,369 6 View

Could a transfected promoter be susceptible to epigenetic modifications?

I am trying to understand if the promoter that I want to stably transfect into the cells through the use of a plasmid could be epigenetically modified, as occurred for the endogenous promoter. Thanks.

26 October 2014 3,353 6 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

What are the effects of temperature and Relative Humidity in the dropwise condensation?

Hello Everyone! I was looking for the effects of the temperature and the relative humidity on the dropwise condensation. I was looking for the papers related to it explaining it in deep. I looked...

12 February 2021 4,196 2 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Coupled hydric and mechanical equation COMSOL?

hello i am trying to study the mechanical deformation due to the humidity on a 3d volume formed of 2 phases. my equation are dw/dt =grad(Dm*div(w)) and the mecanical is sigma=E*beta*nabla(w)....

27 January 2021 5,201 1 View