RNA extraction from yeast showing only one band and no smears found. What could be the possible reasons?

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Abrar Jamous

its look like the RNA is completely degraded! you need to re-extract the RNA.what protocol did you use to extract the RNA?

Md. Mahiuddin Zahangir

Hi Kavilasha Venugopal

It seems the RNA is degraded. Did you check the A260/280 ratios??

Thanks

Kavilasha Venugopal its good method we use it in our lab . be sure to use RNase DNase free tubes and to use DEPC-treated water to dissolve the RNA pellet ,also the 70% ethanol to be prepared using DEPC water .

Good luck