RNA extraction from yeast showing only one band and no smears found. What could be the possible reasons?

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Abrar Jamous

its look like the RNA is completely degraded! you need to re-extract the RNA.what protocol did you use to extract the RNA?

Kavilasha Venugopal

The trizol method

Md. Mahiuddin Zahangir

Hi Kavilasha Venugopal

It seems the RNA is degraded. Did you check the A260/280 ratios??

Thanks

Kavilasha Venugopal its good method we use it in our lab . be sure to use RNase DNase free tubes and to use DEPC-treated water to dissolve the RNA pellet ,also the 70% ethanol to be prepared using DEPC water .

Good luck