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Questions related from Abrar Jamous
Although the transfection and infection were successful using another transfer plasmid, with PWZL.hygro HRAS G12V we didn't get a good infection on MCF10A cells. We used the 3rd generation of...
03 October 2017 304 3 View
Hello everyone,regarding bisulphate treatment, if the sample contains degraded RNA could this affect the efficiency of DNA conversion ?! and what to do to overcome this problem. as you can see...
09 April 2017 5,564 0 View
Hello every one We had a problem with tissue culture: the cells are not proliferating, the cultured amount of cell stay the same, no growth! And there are changes in the shape of cells. We...
21 February 2017 6,679 7 View
hello every body repeating RT-PCR run using the same cDNA way would the CT value for hUBC ( endogenous control) change ?!! this change the CT value affect the result?and give opposite result each...
25 December 2016 1,381 2 View
Hello every one,I want to measure ribosome biogenesis in cancer cell line, please tell me what are the techniques used for this purpose, the best one? thank you :)
15 November 2016 667 5 View
I want to ask what are the benefit of treating RNA sample with DNase I before cDNA and RT-PCR? how dose that affect result !
21 October 2016 728 3 View
how we can identify CpG for specific gene? pubmed give use the gene sequence from the transcription starting site(TSS) what about the upstream sequence how we can find it?and find CpG island in it ?
21 October 2016 2,701 2 View
I am working on regulation of gene expression of a gene and i want to check the status of it enhancer in cancer cells. can you suggest software to use ,and what are the important things to take...
21 August 2016 4,588 3 View
isopropanol, ethanol failed to react at position five of 5-bromo-2,3-disubsttiued pyrazine at position 2, there is ethanolamine and at position 3, there is chlorine atom . does it relay...
21 November 2014 4,399 3 View
