How to define Corrected Total Cell Fluorescence (CTCF) and what is the Units for it?

Hi, Could some one explain me " What is Corrected Total Cell Fluorescence (CTCF) ? " Does CTCF has any units? Thank you

08 September 2018 1,263 0 View

96 well plates for absorbance at 450nm?

Hello friends, Is it okay to use regular tissue culture/cell culture 96 well plates for measuring absorbance at 450nm? Although the wave length is in visible range I am bit skeptical about that...

06 July 2018 6,399 2 View

Can I use "Lipofectamine" based transfection for px459/px330 in CRISPR ?

Hi, I am using electroporation to transfect 661W cells with px459 plasmid in a crispr study. As I see I am loosing too many cells due to electroporation as i am trying to optimize the protocol. My...

03 April 2018 8,269 1 View

Primers to confirm guide ligation into Px459?

Hi, I am trying to ligate/insert the phosphorylated/annealed guide sequence into the Bbs1 digested CIP treated px459. I am getting the bacterial colonies on the AMP agar plate. but when I am...

02 March 2018 582 7 View

Generation time Vs doubling time?

How the generation time of bacteria varies from doubling time? Could some one help me understand the concept. Thanks

01 February 2018 7,697 6 View

What are crispr cas stable cell lines?

Hi, I have just started to learn/adapt CRISPR system for our research and I found few companies providing CRIPSR stable cell lines. Unfortunately, none of them explained to the level where a...

11 December 2017 2,503 2 View

Why do we need to incubate plasmid and bacterial cell mixture on ice during Transformation?

02 March 2016 9,317 5 View

Is it necessary to dilute Accutase to dissociate cryo preserved mouse neurospheres ?

I am trying to dissociate primary neurospheres (mouse), which are cryopreserved in Liq.N2. I followed the standard procedure given by the supplier and the same procedure was followed in many...

01 February 2015 1,080 0 View

What is single cell biology

08 September 2014 6,161 5 View

Why should we add FBS to the serum free media?

We all know, during few cell culture experiments we use serum free media, we still add FBS (Fetal Bovine Serum) in some cases. We are also aware that FBS provides the growth supplements for the...

07 August 2013 3,298 30 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View