Hi, Could some one explain me " What is Corrected Total Cell Fluorescence (CTCF) ? " Does CTCF has any units? Thank you
HI, We all know by literature by adding FCCP we can uncouple the mitochondrial membrane potential and increase the rate of respiration. When I tried/ titrated with a cell line, instead of increase...
31 December 2018 6,737 1 View
Hello friends, Is it okay to use regular tissue culture/cell culture 96 well plates for measuring absorbance at 450nm? Although the wave length is in visible range I am bit skeptical about that...
06 July 2018 6,555 2 View
Hi, I am using electroporation to transfect 661W cells with px459 plasmid in a crispr study. As I see I am loosing too many cells due to electroporation as i am trying to optimize the protocol. My...
03 April 2018 8,373 1 View
Hi, I am trying to ligate/insert the phosphorylated/annealed guide sequence into the Bbs1 digested CIP treated px459. I am getting the bacterial colonies on the AMP agar plate. but when I am...
02 March 2018 681 7 View
How the generation time of bacteria varies from doubling time? Could some one help me understand the concept. Thanks
01 February 2018 7,961 6 View
Hi, I have just started to learn/adapt CRISPR system for our research and I found few companies providing CRIPSR stable cell lines. Unfortunately, none of them explained to the level where a...
11 December 2017 2,629 2 View
During Transformation, before heat shock at 42 degrees for 60-90 sec we keep the plasmid and bacterial cell mixture on ice for 30 min. Will some one help me why we do that? Thanks in advance
02 March 2016 9,586 5 View
I am trying to dissociate primary neurospheres (mouse), which are cryopreserved in Liq.N2. I followed the standard procedure given by the supplier and the same procedure was followed in many...
01 February 2015 1,192 0 View
Dear Friends, I came across a question from my superior "what is single cell biology". I tried answering him saying that studying the biology of cells that are obtained from a single cell and he...
08 September 2014 6,295 5 View
We all know, during few cell culture experiments we use serum free media, we still add FBS (Fetal Bovine Serum) in some cases. We are also aware that FBS provides the growth supplements for the...
07 August 2013 3,594 30 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.
07 August 2024 9,507 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,973 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...
01 August 2024 2,614 1 View
How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...
30 July 2024 3,229 2 View
The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...
30 July 2024 3,126 0 View
I am writing to request a correction regarding the affiliation of one of my co-authors, Donald Ho. Currently, the author's profile incorrectly lists an affiliation with The Hong Kong Polytechnic...
28 July 2024 6,823 0 View