Reverse effect of FCCP in respirometry?

HI, We all know by literature by adding FCCP we can uncouple the mitochondrial membrane potential and increase the rate of respiration. When I tried/ titrated with a cell line, instead of increase...

31 December 2018 6,629 1 View

96 well plates for absorbance at 450nm?

Hello friends, Is it okay to use regular tissue culture/cell culture 96 well plates for measuring absorbance at 450nm? Although the wave length is in visible range I am bit skeptical about that...

06 July 2018 6,399 2 View

Can I use "Lipofectamine" based transfection for px459/px330 in CRISPR ?

Hi, I am using electroporation to transfect 661W cells with px459 plasmid in a crispr study. As I see I am loosing too many cells due to electroporation as i am trying to optimize the protocol. My...

03 April 2018 8,269 1 View

Primers to confirm guide ligation into Px459?

Hi, I am trying to ligate/insert the phosphorylated/annealed guide sequence into the Bbs1 digested CIP treated px459. I am getting the bacterial colonies on the AMP agar plate. but when I am...

02 March 2018 582 7 View

Generation time Vs doubling time?

How the generation time of bacteria varies from doubling time? Could some one help me understand the concept. Thanks

01 February 2018 7,697 6 View

What are crispr cas stable cell lines?

Hi, I have just started to learn/adapt CRISPR system for our research and I found few companies providing CRIPSR stable cell lines. Unfortunately, none of them explained to the level where a...

11 December 2017 2,503 2 View

Why do we need to incubate plasmid and bacterial cell mixture on ice during Transformation?

02 March 2016 9,317 5 View

Is it necessary to dilute Accutase to dissociate cryo preserved mouse neurospheres ?

I am trying to dissociate primary neurospheres (mouse), which are cryopreserved in Liq.N2. I followed the standard procedure given by the supplier and the same procedure was followed in many...

01 February 2015 1,080 0 View

What is single cell biology

08 September 2014 6,161 5 View

Why should we add FBS to the serum free media?

We all know, during few cell culture experiments we use serum free media, we still add FBS (Fetal Bovine Serum) in some cases. We are also aware that FBS provides the growth supplements for the...

07 August 2013 3,298 30 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View