What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Do I need to randomise samples in 96 well plate?

It's been recommended that I randomise samples across a 96 well plate, but not true randomisation, just don't run duplicate samples next to one another. Can someone point me in the direction of...

05 August 2020 7,510 3 View

What are the best emotional intelligence scales that contain subdimensions?

We are looking to do a study on leadership and we want to use a an Emotional Intelligence Scale that meets the following criteria 1. is used in published academic research, not just consulting...

14 July 2020 7,122 2 View

Total of polyphenols in tea extracts conc mg/l to mg/g dry tea?

04 March 2020 3,649 7 View

No title

03 December 2019 5,253 1 View

How can I purify nanoparticles of PEG-PLA ?

Hello, I synthesize nanoparticles of PEG-PLA to encapsulate retinol. Once the nanoprecipitation process is completed, I would like to eliminate the unencapsulated active ingredient (retinol). In...

24 November 2019 8,853 2 View

What is the best research method for analysing documentaries?

I am in the middle of my assignment which is a small research project and I need to edit the methodology. My tutor has recommended me to find an hour long documentary online to analyse to do with...

29 July 2019 6,503 6 View

Purification of PEG-PLA ?

Hello, I'm working on PEG-PLA (5000:15000 Da) and I try to purify it after ROP polymerization. In different papers, they use purification by precipitation (usually with MeOH or diethyl ether)...

10 July 2019 386 9 View

Can I perform meta-analysis when the exposure veriable is continous?

I am hoping to undertake my first meta-analysis, where the exposure variable it mean number of decayed, missing or filled teeth)and the outcome is BMI category (underweight, normal weight,...

09 May 2019 8,757 4 View

Best method to amplify the signal in immunohistochemistry?

I am currently doing immunohistochemistry to look at a membrane antigen, on fixed embedded samples. I want to amplify the signal (low) I am getting, and was wondering which kind of amplification...

24 April 2019 5,263 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

How to reduce or increase CFU/ml value ?

I collected the water sample from pond and 1 ml of pond sample diluted with 9ml with water and 100 microliter was cultured into the MacConkey agar plates .Finally,80 colonies were formed in the...

24 January 2021 1,925 3 View