A picture of a bone slice of mouse femur with bone marrow being slightly red.
Hi Xudan. Oh I forgot to tell, but I'm using the Cryostat for bone slicing at 25 micrometers.
There could be several reasons for a large gap between the bone marrow and the cortex of a mouse femur bone slice: Processing Artifacts: During the preparation of the bone slice for microscopy, there may have been technical issues such as improper fixation, decalcification, or sectioning, leading to separation of the bone marrow from the cortex. Bone Resorption: It's possible that there has been bone resorption, where the bone marrow cavity expands due to the removal of bone tissue by osteoclasts, creating a gap between the bone marrow and the cortex. Pathological Conditions: Certain pathological conditions such as osteoporosis, osteomyelitis, or bone tumors can affect the structure and integrity of the bone, leading to the formation of gaps between the bone marrow and the cortex. Age-related Changes: Aging can also affect bone structure, leading to changes in bone density and architecture, which may result in the formation of gaps between the bone marrow and the cortex. To determine the specific cause of the gap, further examination, possibly including additional imaging or histological analysis, may be necessary.
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