What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Do I need to randomise samples in 96 well plate?

It's been recommended that I randomise samples across a 96 well plate, but not true randomisation, just don't run duplicate samples next to one another. Can someone point me in the direction of...

05 August 2020 7,510 3 View

What are the best emotional intelligence scales that contain subdimensions?

We are looking to do a study on leadership and we want to use a an Emotional Intelligence Scale that meets the following criteria 1. is used in published academic research, not just consulting...

14 July 2020 7,122 2 View

Total of polyphenols in tea extracts conc mg/l to mg/g dry tea?

04 March 2020 3,649 7 View

No title

03 December 2019 5,253 1 View

How can I purify nanoparticles of PEG-PLA ?

Hello, I synthesize nanoparticles of PEG-PLA to encapsulate retinol. Once the nanoprecipitation process is completed, I would like to eliminate the unencapsulated active ingredient (retinol). In...

24 November 2019 8,853 2 View

What is the best research method for analysing documentaries?

I am in the middle of my assignment which is a small research project and I need to edit the methodology. My tutor has recommended me to find an hour long documentary online to analyse to do with...

29 July 2019 6,503 6 View

Purification of PEG-PLA ?

Hello, I'm working on PEG-PLA (5000:15000 Da) and I try to purify it after ROP polymerization. In different papers, they use purification by precipitation (usually with MeOH or diethyl ether)...

10 July 2019 386 9 View

Can I perform meta-analysis when the exposure veriable is continous?

I am hoping to undertake my first meta-analysis, where the exposure variable it mean number of decayed, missing or filled teeth)and the outcome is BMI category (underweight, normal weight,...

09 May 2019 8,757 4 View

Best method to amplify the signal in immunohistochemistry?

I am currently doing immunohistochemistry to look at a membrane antigen, on fixed embedded samples. I want to amplify the signal (low) I am getting, and was wondering which kind of amplification...

24 April 2019 5,263 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View