I need to prepare Na2HPO4/Citrate buffer pH 5.0, it is possible to prepare 1M, 0r 0.5M if yes, please how? Thank you
I want to crystallize my protein with this buffer? i need 0.1M working concentration.
After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...
29 July 2024 9,753 3 View
The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.
25 July 2024 9,976 3 View
I have seen two kinds of explanations given for the phenomenon that I try to draw in the attached png document. One explanation says that an air bubble creates the experience of a solid black...
16 June 2024 6,493 1 View
Cross-slip, twinning and fracture are major deformation modes adopted by loaded materials. It appears sound that these apparently different deformation mechanisms can be analysed on the equal manner!
15 June 2024 8,477 3 View
The terms Fictional Language, Fictitious Language, Artificial Language, or Constructed Language in fiction has been used interchangeably in papers that I have read. Are there differences in these...
05 June 2024 3,453 7 View
Investigating use of Sorghum as part of Regenerative Agriculture management system using cover crops & mower-crimping, Alley Cropping, sheep manure in a zero till management system with...
18 April 2024 2,389 3 View
I'm conducting a multiple case study, and I'm interested in exploring a phenomenon from the perspectives of male and female participants.
14 April 2024 4,352 1 View
The following are the details of the conference: Name of event: International Conference on Community Health Nursing and Public Health (ICCHNPH) Venue: Brno, Czech Republic Date: 9th -10th...
25 March 2024 996 1 View
For cell culture , if want to study about the protein or gene expression ? The treatment need to dilute with medium without fbs & pen strep or complete medium. What happen , if the treatment...
19 March 2024 399 2 View
Hello everyone, Is there anyone who has cloned with pAcGP67A secretion vector with GP67A signal peptide. I tried to clone with insert 1.2kb in the vector, MCS: BamHI and NotI. C-term Thrombin and...
12 December 2023 5,091 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View