Melonney Patrick

5 Questions 2 Answers 0 Followers

Questions related from Melonney Patrick

Regarding Sample Treatment in cell culture ?

For cell culture , if want to study about the protein or gene expression ? The treatment need to dilute with medium without fbs & pen strep or complete medium. What happen , if the treatment...

20 March 2024 400 2 View

Im study on natural product testing on disease,my question why the level of protein expression is not always directly proportional to gene expression?

Which is more important protein or gene expression?

04 October 2023 7,713 4 View

Why my no template control (NTC) for reference gene (GAPDH) amplified ?

Hello , it about my no template control (NTC) result for reference gene (GAPDH). I already run many times and use different batch of reference gene (GAPDH). I also change the master mix and...

01 April 2022 1,249 3 View

How many conc.that I need to use for PMA (phorbol 12-myristate-13-acetate) induced in THP-1 cell culture from monocytes to macrophages?

I will use 6 well plates. I'm studying the migration of the macrophages? Is it possible cell be migrate after it cell already differentiate monocytes into macrophages?

01 January 1970 7,055 2 View

PMA induced in THP-1 cell culture

Hello , i have 1 question ? I study the migration of macrophages. Can the cells migrate after the THP-1 cell induced with PMA (phorbol 12 myristate-13-acetate).

01 January 1970 5,793 2 View