I am doing refolding of light and heavy chain of antibody fragments individually. Want to get information on pathways of tertiary and secondary structure formation during refolding process. So after diluting inclusion bodied I am collecting time interval samples. Can I do directly Fluorescence and CD or I needed some other ways for sample preparations? As Arginine bind with side chain of tryptophan and intrinsic Fluorescence is monitored tryptophan emission.

One thing I am not clear that why people are studying denaturation study while in case of therapeutic protein renaturation is only productive way fir the formation of refolded native protein.