Kayanat Hada

3 Questions 2 Answers 0 Followers

Questions related from Kayanat Hada

What negative delta G value indicate in protein denaturation study?

How conformational changes are occurring in protein denaturation related to the Gibbs free energy changes of that particular protein? For example I have one protein having two domain in its native...

01 November 2021 5,774 2 View

Refolding buffer components like arginine, sorbitol, and redox couple interfere in spectroscopic analysis like Fluorescence and CD during refolding?

I am doing refolding of light and heavy chain of antibody fragments individually. Want to get information on pathways of tertiary and secondary structure formation during refolding process. So...

08 August 2020 8,592 0 View

Why I am getting 4 Bands on gel for single plasmid extraction ?

I extract Plasmid DNA by using QIAGEN Mini Kit. I was hoping to get one clear band at 5 Kb ( My Plasmid Size is 5kb). I used overnight grow culture and then isolate plasmid using QIAGEN Kit. Here...

30 August 2016 321 5 View