Reference gene for normalization of candidate gene expression levels using RT-PCR in hypoxia exposed samples ?

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What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Does behavior (shy vs bold; rare vs abundant) and flock size (small vs large) of bird species play role in niche partitioning among sympatric species?

Sympatric birds may avoid competition via multiple strategies - by different activity period, different diet, different microhabitat etc. Has any one looked into behavior of sympatric birds as...

11 January 2021 9,600 1 View

Which type of polymer is used in base boards of Printed Circuit Boards (PCBs)?

I want to know about the type of polymers in PCBs.

25 May 2020 9,640 15 View

Dear Peers! Could you please oblige me with your knowledge on vulture?

I am looking for proverbs and idioms or any short story mentioning vulture in any language or locality with English translation.

24 February 2020 2,004 9 View

I am supposed to develop a psychometric employability assessment. Any literature or advice that could help me? Also, how do I ensure honest answers?

I have never done this before. So far I have collected scales for different factors after detailed research. I plan on taking items from them and maybe restructuring them to make a proper...

31 January 2020 9,923 2 View

Which funding agency in India will grant traveling allowance for international conference in finance ??

I get an opportunity to present my paper in international conference on June 2020. I am research scholar in management (finance department).Kindly let me know which funding agency will grant...

07 January 2020 7,040 1 View

Can anyone suggest me a solver for binary linear multiplicative programming problem?

The example case is as follows: Maximize(a1 * X1 + a2 * X2 + a3 * X3 + a1 * a2 * Y12 + a1 * a3 * Y13 + a2 * a3 * Y23) constraints are: a1, a2, a3 are binary variable belongs to [0, 1], and a1 + a2...

23 December 2019 5,912 4 View

How can we estimate Nitrogen (N) in sugarcane field using satellite imagery?

I want to estimate nitrogen (N) content in the sugarcane fields using sentinel-2 optical data (Multi spectral Imagery). I have read few papers where simulated values helped in potatoes and other...

16 May 2019 4,580 6 View

How can we download the all the clean drug like compounds from Zinc12 database?

http://zinc.docking.org/subsets/drug-like When I click on sdf/mol2 format , it gives me usual.sdf.bat or usual.mol2.bat. How I will use these file to retreive the compounds????

08 April 2019 2,042 1 View

Bacterial Growth Concern ?

hi All , i am using Rhizobium species for Atrazine (as nitrogen source )removal in MS medium, unfortunately from last week its not showing growth in liquid medium while some bacterial colonies are...

25 February 2019 420 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View