In investigation possible sex differences the important consideration taking into experiment female rats with proper distribution of estrous cycles. The wright way to estimate estrous cycle is to take vaginal smears on daily basis for the 4-5 consecutive days. This handling is known to disrupt stress parameters and influence on activity of HPA axis.
The question is how long it takes to return these values, such as CORT, on the basal levels before handling and taking estrous cycle? What is the recovery time?
Tnx Ivana for the reply.
Did you came across of some references that document the retrieval/normalization of some stress parameters, such as CORT levels, after period of recovery or rest?
zz Milos
Tnx Ivana,
The second paper is great, its what I have been looking for. Although in the section above he mentions that there are reports that show a 30% increase in CORT values.
Kind regards :-)
Milos
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