Hi there..this is my first time to handle reagents..we just bought Hydrocortisone and EGF from Sigma for our cell culture project
problem is i can't figured out how to reconstitute them, the vial doesnt come with any manual, sigma webpage only said to add absolute ethanol (Hydrocortisone) and acetic acid (EGF)
somebody has a tutorial? my working solution should be 10 ug/ml (Hydrocort) and 10 ng/ml (EGF)
doesn't ethanol and acetic acid harmful for cell?
thanks a lot !
let say i have 10 mg of powdered hidrocort, sigma instruct to reconstitute in EtOH and dilute in sterile medium..
if i add 10 ml of EtOH and dilute with 10 ml of Sterile culture medium to achieve 500 ug/ml stock solution, aliquote and stored in - 20 C ? is this procedure alright?
other intruct to add 0,1 % BSA...means?
check for any published paper for their solubility and stock concentration.
ethanol does effect the vialibity in cell culture. so better work on this thoroughly and make a stock
just wondering if i aliquote and freeze reconstitute HC in EtOh, without further dilution using medium, will it be ok ? i plan to dilute it later after thawing..
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