reconstitution tutorial

Hi there..this is my first time to handle reagents..we just bought Hydrocortisone and EGF from Sigma for our cell culture project problem is i can't figured out how to reconstitute them, the...

08 September 2010 9,405 5 View

setting up new lab

hi evryone.. i am setting up a new lab for cell culture, does anyone know what is the composition of Oxygen, CO2 and N2 inside the incubator

06 July 2010 2,229 7 View

Omnitray

Hi evryone, my lab wants to used omnitray for the first time..unfortunately were hurried by deadline and unfortunately the company principal in our country couldn.t even promised the omnitray...

06 July 2010 9,590 0 View

isolating basal keratinocyte

Hi I am newbie on this topic, I am working with skin samples, we try to isolate basal keratinocyte, i found trypsinization with trypsin/EDTA 0,05% and 0,1% is not good enough, also I wonder how to...

01 January 1970 6,491 4 View

Keratinocytes culture problem

I have succesfully isolated and cultured human basal keratinocytes from foreskin for about a year now. Lately I encountered a puzzling problem - for the last isolation I managed to isolate the...

01 January 1970 9,792 18 View

Transdifferentiation

I am trying to transdifferentiate keratinocytes to neurons. Is it possible and what factor should I use?

01 January 1970 7,433 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View