Indra Kusuma

7 Questions 36 Answers 0 Followers

Questions related from Indra Kusuma

reconstitution tutorial

Hi there..this is my first time to handle reagents..we just bought Hydrocortisone and EGF from Sigma for our cell culture project problem is i can't figured out how to reconstitute them, the...

09 September 2010 9,443 5 View

setting up new lab

hi evryone.. i am setting up a new lab for cell culture, does anyone know what is the composition of Oxygen, CO2 and N2 inside the incubator

07 July 2010 2,257 7 View

Omnitray

Hi evryone, my lab wants to used omnitray for the first time..unfortunately were hurried by deadline and unfortunately the company principal in our country couldn.t even promised the omnitray...

07 July 2010 9,635 0 View

Keratinocytes culture problem

I have succesfully isolated and cultured human basal keratinocytes from foreskin for about a year now. Lately I encountered a puzzling problem - for the last isolation I managed to isolate the...

01 January 1970 9,831 18 View

Transdifferentiation

I am trying to transdifferentiate keratinocytes to neurons. Is it possible and what factor should I use?

01 January 1970 7,475 4 View

isolating basal keratinocyte

Hi I am newbie on this topic, I am working with skin samples, we try to isolate basal keratinocyte, i found trypsinization with trypsin/EDTA 0,05% and 0,1% is not good enough, also I wonder how to...

01 January 1970 6,525 4 View

PBMC reaction in coculture?

We cocultured human epidermal keratinocytes with allogeneic PBMC in Human Keratinocyte Growth Supplement (HKGS), Epilife medium, Dulbecco's Modified Eagle Medium (DMEM) with autologous serum...

01 January 1970 4,579 0 View