Recommended extraction of tropoelastin from collagen matrix for use in ELISA?

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MMP-1 Collagen Digestion Protocol?

Does anyone have a protocol recommendation for digesting a collagen matrix with MMP-1?

24 July 2017 8,166 2 View

Benefits of MMP-1 over bacterial collagenase?

I am looking to digest a bovine collagen matrix and MMP-1 continues to be recommended over bacterial collagenase. Is there a specific reason for this?

20 July 2017 5,690 1 View

Cathepsin-K,L,S Collagen Digestion Protocol?

Does anyone have a protocol recommendation for digesting a collagen matrix with Cathepsin K, L, or S?

06 July 2017 2,255 2 View

Bacterial Collagenase Digestion Protocol?

Does anyone have a protocol recommendation for digesting a collagen matrix with bacterial collagenase?

06 July 2017 1,876 0 View

Α-Amylase for Collagen Digestion Protocol?

Does anyone have a protocol recommendation for digesting a collagen matrix with α-Amylase?

06 July 2017 5,075 0 View

Electronic Notebook and Sample Management System?

My team and I are looking for an ELN system with sample tracking features. We like the ability to save protocols, copy and paste them, and see the work other members of the team are doing. An easy...

18 April 2017 858 2 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

The complex interactions between oral microbiota and oral mucosal immunoregulation: implications for oral health and disease development?

This research question explores the complex interactions between oral microbes and oral mucosal immune regulation. The diversity and function of oral microbes have important impacts on oral health...

09 July 2024 7,883 0 View

What is the best approach to culture a mixed immune cell population for at least 48h?

I want to isolate and culture all immune cells from the adipose tissue to isolate EVs. What is the best approach to make sure all cells survive for at least 48h?

03 July 2024 6,501 1 View

Quantification of CD8 positive cells using ImageJ software?

Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...

01 July 2024 8,964 1 View

How do I prepare rat cardiomyocyte cell lysate for ELISA via freeze-thaw method?

Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?

23 June 2024 2,264 4 View

How do you calculate sample size for a clinical trial when you only have cross-sectional data of the outcome parameter?

Hello, I have cross-sectional data on immune cell density in a disease group. Compared to controls, the disease group shows an increase in immune cell density. Now, I want to run a clinical...

17 June 2024 6,257 3 View

What is considered a biological replicate when you are doing an ELISA plate measurement with different cell types?

If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...

17 June 2024 6,705 1 View

Different interpolated concentrations?

I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...

15 June 2024 5,109 1 View

Faisal A. Alshamiry

http://www.sciencedirect.com/science/article/pii/S0142961209005389

http://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.2004.tb09861.x/full

Guido Vogt

Hi,

I use this kit. It's a colorimetric assay but it works fine for skin tissue and cells.

http://www.biocolor.co.uk/product/fastin-elastin-assay/

Have fun