Cathepsin-K,L,S Collagen Digestion Protocol?

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MMP-1 Collagen Digestion Protocol?

Does anyone have a protocol recommendation for digesting a collagen matrix with MMP-1?

24 July 2017 8,166 2 View

Benefits of MMP-1 over bacterial collagenase?

I am looking to digest a bovine collagen matrix and MMP-1 continues to be recommended over bacterial collagenase. Is there a specific reason for this?

20 July 2017 5,690 1 View

Bacterial Collagenase Digestion Protocol?

Does anyone have a protocol recommendation for digesting a collagen matrix with bacterial collagenase?

06 July 2017 1,876 0 View

Α-Amylase for Collagen Digestion Protocol?

Does anyone have a protocol recommendation for digesting a collagen matrix with α-Amylase?

06 July 2017 5,075 0 View

Electronic Notebook and Sample Management System?

My team and I are looking for an ELN system with sample tracking features. We like the ability to save protocols, copy and paste them, and see the work other members of the team are doing. An easy...

18 April 2017 858 2 View

Recommended extraction of tropoelastin from collagen matrix for use in ELISA?

Does anyone have ideas on the best method of extracting tropoelastin from a collagen matrix for use in an ELISA assay? I have tried collagenase, pepsin, oxalic acid, RIPA, and T-PER but they all...

03 April 2017 4,605 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View