Does anyone have a protocol recommendation for digesting a collagen matrix with Cathepsin K, L, or S?
You can contact Dr Manni Luthra Guptasarma, PGIMER Chandigarh, India. Her group work on cathepsin-K.
Any reason why not using collagenase instead?
Does anyone have a protocol recommendation for digesting a collagen matrix with MMP-1?
24 July 2017 8,093 2 View
I am looking to digest a bovine collagen matrix and MMP-1 continues to be recommended over bacterial collagenase. Is there a specific reason for this?
20 July 2017 5,621 1 View
Does anyone have a protocol recommendation for digesting a collagen matrix with bacterial collagenase?
06 July 2017 1,820 0 View
06 July 2017 5,018 0 View
Does anyone have ideas on the best method of extracting tropoelastin from a collagen matrix for use in an ELISA assay? I have tried collagenase, pepsin, oxalic acid, RIPA, and T-PER but they all...
03 April 2017 4,541 2 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
02 March 2021 3,060 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View