Bacterial Collagenase Digestion Protocol?

Alex Mol @Alex_Mol2

07 July 2017 0 2K Report

Does anyone have a protocol recommendation for digesting a collagen matrix with bacterial collagenase?

Badges
Science topic

Similar topics
Mathematics
Algebra
Matrix

More Alex Mol's questions See All

MMP-1 Collagen Digestion Protocol?

Does anyone have a protocol recommendation for digesting a collagen matrix with MMP-1?

24 July 2017 8,166 2 View

Benefits of MMP-1 over bacterial collagenase?

I am looking to digest a bovine collagen matrix and MMP-1 continues to be recommended over bacterial collagenase. Is there a specific reason for this?

20 July 2017 5,690 1 View

Cathepsin-K,L,S Collagen Digestion Protocol?

Does anyone have a protocol recommendation for digesting a collagen matrix with Cathepsin K, L, or S?

06 July 2017 2,255 2 View

Α-Amylase for Collagen Digestion Protocol?

Does anyone have a protocol recommendation for digesting a collagen matrix with α-Amylase?

06 July 2017 5,075 0 View

Electronic Notebook and Sample Management System?

My team and I are looking for an ELN system with sample tracking features. We like the ability to save protocols, copy and paste them, and see the work other members of the team are doing. An easy...

18 April 2017 858 2 View

Recommended extraction of tropoelastin from collagen matrix for use in ELISA?

Does anyone have ideas on the best method of extracting tropoelastin from a collagen matrix for use in an ELISA assay? I have tried collagenase, pepsin, oxalic acid, RIPA, and T-PER but they all...

03 April 2017 4,605 2 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Is it true that $\det(V(A))$ may be only $\pm 1$, depending on $n$, for the last symmetric tridiagonal matrix $A$?

One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...

08 August 2024 6,690 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Request a single Lecture notes for math as detailed as this that I can find in one place?

- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...

03 August 2024 2,366 0 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

Is it true that science is leaving the era of mathematics and entering the era of matrix mechanics?

We assume this to be true. Science leaves the era of mathematics and enters the era of matrix mechanics and the turning point is the discovery of numerical statistical theory called Cairo...

31 July 2024 3,900 2 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Help on understanding the implementation of Mori Tanaka method on MATLAB?

I am new to Micromechanics and having similar problem with understanding the implementation of the formula's. I would appreciate if anyone can guide me on how to go about getting a scalar value...

30 July 2024 969 0 View