Does anyone have a protocol recommendation for digesting a collagen matrix with α-Amylase?
Does anyone have a protocol recommendation for digesting a collagen matrix with MMP-1?
24 July 2017 8,093 2 View
I am looking to digest a bovine collagen matrix and MMP-1 continues to be recommended over bacterial collagenase. Is there a specific reason for this?
20 July 2017 5,621 1 View
Does anyone have a protocol recommendation for digesting a collagen matrix with Cathepsin K, L, or S?
06 July 2017 2,164 2 View
Does anyone have a protocol recommendation for digesting a collagen matrix with bacterial collagenase?
06 July 2017 1,820 0 View
Does anyone have ideas on the best method of extracting tropoelastin from a collagen matrix for use in an ELISA assay? I have tried collagenase, pepsin, oxalic acid, RIPA, and T-PER but they all...
03 April 2017 4,541 2 View
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...
01 March 2021 9,332 6 View
Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...
01 March 2021 2,957 8 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?
28 February 2021 3,864 3 View
How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...
28 February 2021 7,397 8 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View
Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...
28 February 2021 364 3 View
I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...
28 February 2021 5,415 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View