We piloted the D2R primary from Millipore in house sparrow tissue and saw no specific binding. Looking for a D2R primary that has been piloted in a songbird.
Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...
05 August 2024 8,946 3 View
Dear All, I wanted to know if anybody has experience in processing multispectral data from the DJI Mavic 3M using OpenDroneMap? I know, that ODM works with Phantom 4 multispectral data. Are there...
26 May 2024 797 3 View
Socialization has a basis in justice and also in mercy - but how it is possible to recognice the specific importance for human sozialization.
25 March 2024 6,100 0 View
We currently use the MitoViewer for the staining of adipocytes and stromal vascular fraction (SVF). But we have no signal in cells that have formed lipid droplets. Does anyone have any experience?...
04 January 2024 1,755 0 View
Hello all, Does anyone have experience in monitoring fatty acid oxydation in living cells using commercial kits (abcam, FAOblue) ? Is there any trick to allow FAO detection (glucose, serum...
13 September 2023 4,992 0 View
Hello, Have you ever extracted nuclei from fixed samples ? When I fix my HepG2 cells with 1% formaldehyde, I noticed that the fixation prevents cell lysis with regular nuclei isolation buffer,...
03 May 2023 561 5 View
Hi all, I've recently upgraded from the Agilent Seahorse XF24 to the XFe24 and have started having issues with the OCR calibration. The rep suggested that storing the hydrated cartridge at 4C even...
26 January 2023 3,288 3 View
I am having difficulties obtaining a copy of: McHenery J.G. and Mackie C.M., 1999. Revised expert report on the potential environmental impacts of emamectin benzoate, formulated as Slice, for...
07 December 2022 6,678 10 View
Hi, During my polyurethane production once the exotherm is peaking and I want to slow the reaction down I add xylene, but not enough to severely effect the temperature. (IE 200 grams in 10000,...
09 October 2022 5,210 0 View
I recently included GEE models in the statistics and calculated it with the wald chi square test. Does anyone know how to correctly report the findings considering APA-Guidelines? e.g we would...
21 May 2022 9,637 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...
25 July 2024 1,545 3 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View