Hello,
I always prepare fresh lysis buffer(RIPA),whenever I prepare my samples for Western Blot. However,since the volume added to my samples are very less,the rest just becomes a waste. I am curious if we could re-use the lysis buffer added with the protease and phosphatase inhibitors? If,so what is the shelf- life? And,how many time could it be reused? Any suggestions on this would be of great help!
Thanks,
One can freeze and thaw RIPA buffer 2-3 time without losing its potency as long it is stored on ice during its usage (20-30 min). Store the buffer frozen at -80 degree C.
I usually prepare 50 mls of RIPA and aliquot in 1ml eppendorfs for freezing at -20. I freeze/thaw these aliquots maybe 3-5 times without any issues.
I generally add a tablet each of protease and phosphatase inhibitors to 10 mL of RIPA buffer and store in 4 degrees. Is this a correct way of storing? (P.S: Each tablet is recommended for 10 mL of buffer)
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