19 September 2017 3 668 Report

I am growing RAW264.7 macrophages in DMEM in a T-75 (10mL) flask. It has been 2 weeks but the cells haven't crossed 1 million/mL count. The viability is 80%+. On the other side, my JURKAT cells have crossed 1million/mL count within 2 days so I can be sure I am not doing anything wrong with my handling. 

What should I do to hasten the growth of the macrophages? Should I add 0.0004% B-mercaptonol to take care of any ROS that may be produced by the macrophages? I am following the nutritional requirement as shown on the ATCC page.

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