Jatin Sharma

5 Questions 5 Answers 0 Followers

Questions related from Jatin Sharma

I am trying to cross link ASC protein using DSS to make ASC oligomers. Is there a possibility for non-asc oligomerization?

I have taking the cell lysate and adding DSS which cross links primary amines. Since I haven't purified ASC, what are the odds that I would find ASC getting cross linked to other non-asc proteins...

07 November 2017 6,111 2 View

RAW264.7 macrophages are growing extremely slow in DMEM. How can I hasten the growth?

I am growing RAW264.7 macrophages in DMEM in a T-75 (10mL) flask. It has been 2 weeks but the cells haven't crossed 1 million/mL count. The viability is 80%+. On the other side, my JURKAT cells...

19 September 2017 688 3 View

RMSD plot doesn't converge?

I am trying to simulate a protein bound to a ligand but I can't see the point of convergence in the RMSD plot. The protein I am using has some chains missing. Could that be the cause?

03 August 2016 6,821 2 View

My TBST buffer shows some cloudiness after the addition of primary antibodies to the Nitrocellulose membrane. Can it be a fungal growth?

I am performing a Western blot for the NLR adapter ASC protein but during the primary antibody addition, there is some turbidity in the buffer. I am sure this is not a precipitation reaction. What...

24 June 2016 7,325 3 View

Why tRNA and rRNA don't require 5' mRNA capping for stability like mRNA does?

What stabilises these RNAs?

02 June 2016 1,194 1 View