Jatin Sharma

3 Questions 4 Answers 0 Followers

Questions related from Jatin Sharma

RAW264.7 macrophages are growing extremely slow in DMEM. How can I hasten the growth?

I am growing RAW264.7 macrophages in DMEM in a T-75 (10mL) flask. It has been 2 weeks but the cells haven't crossed 1 million/mL count. The viability is 80%+. On the other side, my JURKAT cells...

19 September 2017 666 3 View

RMSD plot doesn't converge?

I am trying to simulate a protein bound to a ligand but I can't see the point of convergence in the RMSD plot. The protein I am using has some chains missing. Could that be the cause?

03 August 2016 6,785 2 View

My TBST buffer shows some cloudiness after the addition of primary antibodies to the Nitrocellulose membrane. Can it be a fungal growth?

I am performing a Western blot for the NLR adapter ASC protein but during the primary antibody addition, there is some turbidity in the buffer. I am sure this is not a precipitation reaction. What...

24 June 2016 7,291 3 View