Hello,
I need to buy precast sigma milipore gradient gels (Bis-Tris precast gels mPAGE® 4-20%).
Has anyone ever used them and could share their experience?
Typically, I conducted protein electrophoresis with Tris-Glycine-SDS buffer,
and here the manufacturer recommends MES and MOPS. I guess I shouldn't see a big difference.
I will be grateful for any information. Regards.
Hi,
Bis-Tris precast gels (mPAGE® 4-20%) are commonly used for protein electrophoresis and are known for their high resolution and good separation efficiency. It's great to hear that you're considering using these gels for your protein analysis.
In terms of buffer compatibility, as you mentioned, the manufacturer recommends using MES and MOPS, but Tris-Glycine-SDS buffer is also commonly used for protein electrophoresis. The choice of buffer will depend on the specific requirements of your protein analysis and the characteristics of the protein sample.
It is important to note that switching from Tris-Glycine-SDS buffer to MES/MOPS buffer may affect the migration behavior of your protein, so it is advisable to run a few test gels before using the Bis-Tris gels for your analysis. You can also try using a different buffer system if you are not satisfied with the results obtained with MES/MOPS buffer.
Overall, Bis-Tris precast gels (mPAGE® 4-20%) are a good choice for protein electrophoresis and the choice of buffer system will depend on the specific requirements of your analysis.
I hope this information is helpful.
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