I am looking for input on how to quantify the amount of signal from lung tissue sections stained with anti- alpha SM actin. Is there a program out there that I can upload images of my tissue and label aSM actin for quantification?
You can use QuPath ( https://qupath.github.io/ ) or Fiji imageJ ( https://fiji.sc/ ) for quantitative IHC image analysis. Both softwares are freely available.