We have drugs that are highly insoluble in most everything other than DMSO. We've found that our mice that receive IP injections with DMSO concentrations ranging from 10-30% in PBS become very heavily sedated to the point where we consider euthanasia but not sure if its is a drug or its the DMSO. Has anyone ever experienced such a thing?
Tom Masi
You should not use DMSO at concentrations higher than 10% for in vivo studies. If you can get away with lower than 10% the better. Please see the attached SOP from WSU for information about using DMSO in vivo.
Also, you should have a DMSO alone control group at the same concentration as your drug is resuspended in, such that you can determine any adverse events that occur.
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