T-test or one way ANOVA in RT-qPCR gene expression?

Hello, I checked the expression of several genes from different strains and I obtained the values of the relative normalized expression of each gene from each strain compared to the relative gene...

28 April 2019 3,598 3 View

How do i set up Biological replicates using CFX Maestro Software Bio-Rad?

Hello everybody, I am performing gene expression analysis using the Bio-Rad CFX maestro Software. I have different strains which I set up as "biological groups". In different plates I put my...

13 January 2019 5,685 2 View

How much sample should be used in a no RT control?

I would like to know when running a no rt control for qPCR should the amount of template used be the same as the amount used to make cDNA or the amount of cDNA used in the qPCR. I use 100ng of RNA...

15 October 2018 7,022 3 View

What is the toxicity of Sambucus nigra leaves, shoots and roots? Is the amount of cyanogenic glycosides in a given speciman variable?

Need a clear answer. I am seeing conflicting information on this.

19 October 2016 7,275 7 View

Replacing a standard curve reagent in validated assay?

17 October 2016 5,744 6 View

Copan eNAT swabs for microbiome work?

Hi all, So I've been working up a lab protocol for extracting DNA from oral and nasal swabs for 16S sequencing. Just when I thought I'd made my decision on swabs to use etc I get a recommendation...

26 September 2016 4,651 2 View

Optimising a competitive ELISA for sensitivity?

20 June 2016 8,127 10 View

Does the presence of trypsin degrade cytokines?

I have samples of frozen tissue supernatant that are being analyzed for cytokine production. However, the cells have been treated with trypsin. Will this affect the concentration of cytokines once...

15 June 2016 2,381 6 View

How do I solve my problem with my ChIPs?

01 September 2015 287 5 View

Why do I have flaky DNA clumps upon Ethanol precipitation?

I did a large scale (6 L) extraction of DNA using Phenol/CIA and PEG precipitations. The step before the ethanol precipitation is CIA extraction. After adding the 2.5X ethanol the DNA looks...

19 March 2015 9,510 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View