Hello,
I want to work on miRNA extracted from plasma samples using Qiagen miRNeasy Serum/PlasmaKit (50 from patients with hematological malignancies. I made cDNA synthesis using miRCURY LNA RT Kit For 8–64 cDNA synthesis reactions and after that I want to use Cat NO. A25741 Thermo Fisher Scientific, POWRUP SYBR MASTER MIX,1 ML 1 M to make real time PCR for the panel from Qiagen miRCURY LNA miRNA Focus Panel - Cancer/serum Exosome; 4x 96-plate using Quantstudio 5 device , so Is the master mix from thermo ok to used with these components?
The PowerUp SYBR Green Master Mix from Thermo Fisher Scientific is a commonly used reagent for real-time PCR, and it is compatible with a variety of real-time PCR instruments, including the QuantStudio 5 from Thermo Fisher.
However, when using different kits and reagents from different manufacturers, it's important to ensure compatibility and optimize the conditions for your specific experiment. Here are some considerations:
Before proceeding with the large-scale analysis of patient samples, it's advisable to perform a pilot experiment with a subset of samples to optimize the assay conditions and validate the performance of the primer sets, cDNA synthesis, and real-time PCR using the PowerUp SYBR Green Master Mix.
Always follow the recommended protocols provided by the respective manufacturers for each kit and reagent. If you encounter any issues or have specific questions about compatibility, you may also consider reaching out to technical support from both Qiagen and Thermo Fisher Scientific for guidance.
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