Lugien Al-khalaileh

5 Questions 8 Answers 0 Followers

Questions related from Lugien Al-khalaileh

Analysis of real time pcr data ?

1. As I navigate through the complexities of miRNA expression analysis using qPCR, my study involves a panel of 20 plates, each containing 96 wells—18 dedicated to patient samples and 2 for...

14 January 2024 5,334 4 View

QIAGEN MIRCURY LNA MIRNA FOCUS PANEL - CANCER with POWRUP SYBR MASTER MIX - THERRMO Ist possible to use them together?

Hello, I want to work on miRNA extracted from plasma samples using Qiagen miRNeasy Serum/PlasmaKit (50 from patients with hematological malignancies. I made cDNA synthesis using miRCURY LNA RT...

26 November 2023 4,262 1 View

How i can measure the integrity of MiRNA? I was used Qubit4 fluormeter ,but I cant get any result ?

I'm working on MiRNA research and I extracted miRNA by using a serum/plasma Qiagen kit specific for MiRNA extraction because my samples are plasma from Leukemia/Lymphoma patients. After that, I...

03 October 2023 1,856 4 View

In what degree microRNA affected by hemolysis? And what if the sample is slightly hemolyzed ?

Source of samples Plasma to extract microRNA

30 March 2023 7,052 2 View

Is microRNA affected by blood transfusion?

Can we collect samples from patient take blood transfusion to measure microRNA?

21 March 2023 6,122 4 View