Yamak Mehta

2 Questions 60 Answers 0 Followers

Questions related from Yamak Mehta

How to remove DNA from protein solution (Protein expressed extra-cellular in E.coli and pI is ~ 5.5) with chromatography or other techniques ?

Although two ion exchange chromatography ( CEX and AEX) is in place in bind and elute mode ( NaCl conc. < 200 mM in both case for elution) still DNA removal is not sufficient and it is more than...

05 May 2014 9,632 8 View

Compare the purification of monoclonal antibodies from cell culture supernatant with metal–chelate CIM monolithic columns (IgG1 mAbs) and Protein Affinity Chromatography.

1. It can be an alternative to Protein A Chromatography. 2. Is it more cost effective than Protein A Chromatography. 3. What are the advantages & disadvantages of this techniques with respect to...

03 March 2012 8,933 18 View