Pull down assay? - FAQS.TIPS

08 August 2016 0 5K Report

Hi,

I want to study protein protein interaction based on pull down assay. I want to know how to generate antibody against my target protein?

Badges
Science topic

More Surjit Singh's questions See All

Is it possible that point mutation does not change the gene and protein expression but it affects the protein function?

Hello, I am working with a kind of mutated protein and it seems that at the transcriptional level there is no change in the gene expression compared to wild type where as in western blot I can...

31 December 2018 5,786 2 View

Does DH5 alpha carry T7 and SP6 promoter region?

Hello everyone, I am trying to clone 300bp of DNA in DH5 alpha but after clonin i saw double band on the gel. one the desiredband which i need to get and another band just above my desired band....

10 November 2015 9,738 4 View

Is it necessary to clear PhD enterance examintions to get enrolled in PhD programs?

I would like to ask whether is it applicable to judge a student integrity and knowledge on the basis of these enterance test conduct by the educational system of India to get admission in PhD...

05 June 2014 3,117 6 View

What are the different types of analysis that can be done using a 16S rRNA gene sequence apart from phylogenetic tree analysis?

I have three 16S RNA gene sequences of three different bacteria. I have done the phylogenetic analysis and also constructed its dendrogram. But I want to know whether anything else can be done...

02 March 2014 4,037 1 View

What is the best way to remove bacteria from soil?

I want to remove bacteria present in the soil. There are options for killing them but killing will not eradicate bacteria from soil. So, I would like to know whether anybody can suggest to me how...

01 February 2014 6,016 8 View

How to detect major antibiotic groups by chemical reaction?

I want to do qualitative analysis of different antibiotic present in a mixture

02 March 2013 7,875 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View