Hi,
I want to study protein protein interaction based on pull down assay. I want to know how to generate antibody against my target protein?
Hello, I am working with a kind of mutated protein and it seems that at the transcriptional level there is no change in the gene expression compared to wild type where as in western blot I can...
31 December 2018 5,909 2 View
Hello everyone, I am trying to clone 300bp of DNA in DH5 alpha but after clonin i saw double band on the gel. one the desiredband which i need to get and another band just above my desired band....
10 November 2015 9,881 4 View
I would like to ask whether is it applicable to judge a student integrity and knowledge on the basis of these enterance test conduct by the educational system of India to get admission in PhD...
05 June 2014 3,218 6 View
I have three 16S RNA gene sequences of three different bacteria. I have done the phylogenetic analysis and also constructed its dendrogram. But I want to know whether anything else can be done...
02 March 2014 4,150 1 View
I want to remove bacteria present in the soil. There are options for killing them but killing will not eradicate bacteria from soil. So, I would like to know whether anybody can suggest to me how...
01 February 2014 6,158 8 View
I want to do qualitative analysis of different antibiotic present in a mixture
02 March 2013 7,968 1 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View