Dear Tejal,

Have you grown the bacteria on MHA(Mueller Hinton agar)? Try this it will surely releases the pigment pyocyanin and will gives a fluorescent green colored growth. 

Dear Ankita,

i have grown Pseudomonas on MH agar & cetrimide agar as well, it shows good pigmentation. But not on basic media like Nutrient agar. Can u tell what can be done to enhance its pigmentation?

Prepare nutrient agar by adding agar(1.5%) in nutrient broth (Do not use the nutrient agar media directly). After streaking with the inoculum incubate the plates at room temperature for 48hrs. It will surely gives the desired results.

Good luck.

P. aeruginosa generally pigments well on sheep blood agar incubated at 37 C

As Tejal said "Cetrimide agar" will give good growth of P. aeruginosa , in one of my study also i have used cetrimide agar it has given good result. 

Thank you all for your rsponse , it helped me achieve good results.