Protocol to assay sulfated proteoglycans in adherent cells?

29 February 2024 1 937 Report

Does anyone have a protocol for a colorimetric assay that uses Alcian Blue or DMMB to compare sulfated proteoglycan content in adherent cancer cell lines?

Malcolm Nobre

Dear Dr. Dylan Glubb

You may want to refer to the article attached below. Please see the protocol under ‘Sulphated Glycosaminoglycan Assay’.

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0013002

Regards,

Malcolm Nobre

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