29 February 2024 1 915 Report

Does anyone have a protocol for a colorimetric assay that uses Alcian Blue or DMMB to compare sulfated proteoglycan content in adherent cancer cell lines?

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Where can I find data sets containing the average mass of terrestrial predators and their prey?

For my final year thesis I require data on the average body mass of terrestrial predators and their prey. I have come across many papers which have used such data sets however, the data is not...

04 February 2021 8,348 3 View

What are the best websites to find international PHD programs?

As the title implies, I am struggling to find good resources for when it comes to finding international funded research projects outside of the UK within the agricultural and plant sciences....

22 November 2020 2,593 2 View

Can I include multiple outcome measures of the same construct, from the same study, in a meta-analysis?

I am carrying out a meta-analysis that looks at cognitive inhibition as an outcome. A wide range of assessment types can be used for this outcome and sometimes a study will include two or three...

15 November 2020 7,055 1 View

How to analyze data from an augmented design for locations x genotype interaction?

I have data of multiple environment trials (MET) , the design used was an augmented design in each of the locations. And I would like to analyze the data with Rstudio or a free software. Does...

06 November 2020 6,084 3 View

Why did i not getting plasmid band in electrophoresis even when i got pellet after alkali lysis?

Is there any reason for i failed getting any bands on electrophoresis after i did plasmid isolation from E. coli DH5a? My plasmid is ~17 kb in size and i already checked the transformant with...

16 October 2020 6,832 5 View

Pooling multiple continuous variables from the same study?

I am looking to meta-analyze the differential effects of physical abuse and neglect on depression in a meta-analysis. If I come across a study the reports a correlation between a continuous...

24 September 2020 4,211 3 View

What is the correct way to store my ELISA buffers?

Hello I was wondering what the correct storage for the following buffers was and how long they last: Coating buffer: Na2CO3-NaHCO3 pH9.6 Wash Buffer: PBS & 0.05% Tween 20 Antibody...

19 November 2019 4,030 3 View

What pharmaceuticals are present in Irish and European surface waters?

I am doing a research project into the prevalence of pharmaceuticals in surface water and i was looking to see if anyone knew of any up to date articles. Additionally, is their any priority...

02 July 2019 166 6 View

What are current hot research topics in reinforcement/adaptive learning?

What are current hot research topics in reinforcement/adaptive learning?

04 June 2019 3,212 2 View

Will cells induced to senescent exhibit the SASP?

I am attempting to devise a preliminary cell based assay that concerns the impact of SASP factors on uptake of a particular extracellular factor. The assay of uptake already works, however it uses...

24 February 2019 9,310 2 View

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Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Mysterious line across SDS gel?

Does anyone have the similar issue? A line across the denature SDS gel shows up after staining with Coomassie blue and there are many lines in every lane if you take a closer look. This gel had 15...

07 January 2021 5,610 4 View

How to analysis orientation molecule adsorbed on mineral clay molecular dynamics using gromacs?

I'm still confused. Whether to determine the methylene blue orientation, I have to use the order parameter (gmx order) or the gmx angle? I want to determine the orientation of the methylene blue...

31 December 2020 2,386 3 View

How to compare two images obtained from optical microscope?

What program can I use to evaluate two images from an optical microscope, e.g., before and after methylene blue staining. I would like to quantify the degree of staining of cells with a dye....

14 December 2020 9,223 2 View

My Wright-Giemsa Stain on macrophages isn't showing any cytoplasm, just nucleus. Any tips to improve my staining?

I'm pretty sure they are mostly macrophages since after a 2 hour incubation in M199 they are stuck with pseudopodia. My slides just aren't pretty enough for me. I'm using Fisherbrand Wight-Giemsa...

13 December 2020 5,475 3 View

Blue native page transfer problem?

Hello, I am writing this hoping someone can help me. I am just starting out with BN-PAGE and I have a transfer problem. It seems each time I transfer (2 membranes at a time) in a BioRad wet...

09 December 2020 1,008 3 View

Is there any possible stabilize metal-doped blue-emitting light perovskite through self-assembly method?

Now, I have got a metal-doped perovskite in which blue shift happens result. I tried to make it a more blue shift to get a stable and very blue perovskite. So I think is there any possible...

04 November 2020 8,384 3 View

BBB permeability assay in mice ?

Hello! Is there anyone who could recommend a protocol for testing the permeability of the blood brain barrier in mice ? I tried to use these protocols (Assessment of Permeability in Barrier Type...

01 November 2020 8,191 3 View

What are the protocols for H&E Alcian Blue method?

i want to know the protocols for H&E Alcian blue method for studying histology of intestine of fish

26 October 2020 6,351 3 View

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