Our lab has some leftover TRI solution and column-based kit for RNA isolation. Is it possible to use them together? Could i use the TRI solution to homogenize the sample then followed by column purification from the column-based kit?
We work with plant samples with some limited access to refrigerated centrifuge. With reduced centrifugation time i could use the regular centrifuge instead.
You can definitely use Trizol with some column based methods. You can check Trizol plus Purelink RNA extraction kit by invitrogen. In this kit they homogenize with Trizol and follow with using the Purelink columns. It works well with animal tissues, but I have not tried with plants. Also it has a 15 min 4 degree celsius centrifugation step followed by non refrigerated centrifugation.
you can use the TRI solution to homogenize the sample then followed by column purification from the column-based kit. In the absence of a refrigerated centrifuge, act quickly and place the samples and columns in crushed ice during transfers.
Yes its possible to combine organics + spin column for RNA purification, but of course be careful as certain kits/columns/reagents are not fully compatible. There is a product - TRIzol Plus RNA Purification Kit providing a simple method for isolating high-quality total RNA from a wide variety of samples. The kit combines the strong lysis capability of TRIzol Reagent, followed by a silica-column purification protocol from the PureLink RNA Mini Kit, to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.
You can try only one sample, as some components of a kit could interfere with other components of the other kit. Best of luck!
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Column