Multisim summary (Mon Jun 14 14:01:32 2021):
Stage 1 completed.
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Total duration: 0h 1' 20"
Multisim failed.
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I have been trying to get my PCR to work and amplify the correct region but can not figure out what is going on with it. I seemed to have nailed it down to something with the primers. One of the...
19 May 2024 5,087 5 View
Does anyone have a protocol for a colorimetric assay that uses Alcian Blue or DMMB to compare sulfated proteoglycan content in adherent cancer cell lines?
28 February 2024 936 1 View
Hello! I'm conducting an endpoint RT-PCR, and I'm working with MDCK cells (a dog cell line). I'm encountering multiple bands during amplification. I included a negative control (water) as the...
27 December 2023 5,495 3 View
I want to knock out a non-essential gene in endometrial cancer cell lines and am wondering what is the most straightforward approach to do this efficiently. Which is the best CRISPR system to use?...
07 September 2023 246 4 View
I discovered today that getting a premix blocking buffer for phospho proteins is cheaper than getting the BSA to make my own (the only cheaper BSA I found is not recommended for blocking by...
25 October 2022 6,111 0 View
I have been experiencing consistent contamination of the pharmaceuticals ciprofloxacin, trimethoprim, carbamazepine, azithromycin and venlafaxine. The peak height remains the same with or without...
04 June 2022 1,645 8 View
Our lab has some leftover TRI solution and column-based kit for RNA isolation. Is it possible to use them together? Could i use the TRI solution to homogenize the sample then followed by column...
28 November 2021 9,353 4 View
Although pollutant removal by a solid inert surface (substratum) in a bioreactor is normally modelled using adsorption isotherms (Langmuir/Freundlich) due to it being considered a very simple...
30 September 2021 7,554 1 View
Based on Fick's second law of diffusion, the mathematical change in the wastewater concentration over time is represented as "Accumulation = Diffusion - Reaction". Here, the substrate...
27 September 2021 1,669 1 View
I am currently planning out a peat replacement experiment, where I need to calculate the amount of excess Nitrogen Phosphates and potassium that I need to add in order to ensure the growth of the...
25 March 2021 2,308 8 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
Hi, I'm working on heart flow cytometry, and I use anti-mouse CCR2-BV785 (biolegend, cat150621) for staining, 20 minutes, 4 degree. But I couldn't see any CCR2+ cells. So I'm not sure if it's...
03 July 2024 3,969 4 View
Hi all, I am working on subroutines (UMAT and others) with Abaqus. I would need to calculate the average stress by element to eventually change its Young modulus. How should I implement that...
25 June 2024 5,205 1 View
Hi all, Second question on the same or so topic. I am working on subroutines (UMAT and others) for Abaqus to implement Tsai-Wu failure criteria for non-composite structures (I work on long...
25 June 2024 2,955 1 View
I'm researching the ball bearing failure, and could not find the ABLT-1A bearing run-to-failure dataset... If anyone has it, I kindly ask you to forward it to me....
13 June 2024 2,176 0 View
Hi colleagues, I am currently working on shear strengthening of reinforced concrete (RC) beams using CFRP wraps and FRCC jackets. As part of my research, I am performing a finite element method...
11 June 2024 3,670 5 View
Hi, I'm working on the immune cells in the heart and have a problem with CCR2 marker. I did some research and found there's CCR2+ monocytes in heart failure mouse model. When I analysed CCR2...
08 June 2024 7,315 5 View
I am using the discrete fastener method for an LSF frame, and I want to consider the shear failure of rivets in my simulation. However, when I define lower and upper bounds for shear force, Abaqus...
06 June 2024 9,241 0 View
Cartridge is not loading.
03 June 2024 6,560 0 View
Dears, while running nonlinear analysis for 3story building this message appear when analysis complete CONVERGENCE FAILURE OCCURED IN CASE: K6 AT TIME STEP 422, RELATIVE ERROR =...
02 June 2024 6,176 1 View