Dylan Glubb

4 Questions 3 Answers 0 Followers

Questions related from Dylan Glubb

Protocol to assay sulfated proteoglycans in adherent cells?

Does anyone have a protocol for a colorimetric assay that uses Alcian Blue or DMMB to compare sulfated proteoglycan content in adherent cancer cell lines?

29 February 2024 915 1 View

What's the easiest CRISPR approach to efficiently knock out a gene in a cell line?

I want to knock out a non-essential gene in endometrial cancer cell lines and am wondering what is the most straightforward approach to do this efficiently. Which is the best CRISPR system to use?...

08 September 2023 204 4 View

What is the best siRNA approach (e.g. SMARTpool, etc.) to knockdown gene expression?

I've used Dharmacon SMARTpool siRNAs before but I've seen some articles which state that these low complexity pools (of 4 siRNAs) can lead to false positives/negatives. What do people consider the...

27 July 2018 9,994 4 View

ChIP-seq: what fold enrichment is needed from ChIP qPCR before proceeding to sequencing?

I've performed ChIP qPCR on my samples using positive control regions and determined the enrichment relative to negative control regions using the delta delta Ct method. I'm seeing enrichment in...

14 June 2016 9,284 5 View