Salma Batool

Let's say you get silver nanoparticles using tea leaf extract. The capping agent is an extract. The flavonoid extract molecules reduce silver ions to nanoparticles. The remaining molecules are adsorbed on the surface of the nanoparticles and play the role of capping agents. The hydroxyl group of the flavonoid will turn into a quinoid group during the redox reaction and will not significantly affect the mass of the capping agents. Therefore, the solid mass of the extract is acapping agent.

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Thank you Yuri Mirgorod .

Hi Salma Batool

You can use TLC for the separation of the alkaloids and flavonoids from the plant extract. Then purify or separate the particular capping agent. Again characterize the sample via HPLC or GC-MS. Or you may 1st go for the GC-MS and identify the particle alkaloid or flavonoid.

This paper may help you Article Characterization and active component identification of Prem...

Regards

Did you try to bind it with strong ligands such as EDTA??