Dear All,

May I know how do I extract proteins from the stomach of fish? I have been using the commonly used RIPA buffer, but to no avail (the lanes were clean after development)

I have attached a picture of coomasie blue staining for stomach and gills (positive control).

The lanes are as follows: Ladder, Stomach proteins, gills protein. Please disregard the last two lanes.

Please advise.

Thank you!