Mel Veen Boo

5 Questions 9 Answers 0 Followers

Questions related from Mel Veen Boo

Protocol for stomach protein extraction for western blot?

Dear All, May I know how do I extract proteins from the stomach of fish? I have been using the commonly used RIPA buffer, but to no avail (the lanes were clean after development) I have attached...

11 November 2018 1,217 0 View

Please suggest a good protein extraction method for Symbiodinium/Dinoflagellate to be used in Western Blot?

Dear All, I am having problems in extracting the transmembrane proteins from Symbiodinium, but no problems with cytoplasmic proteins. The extraction medium I am using consists of Tris, sodium...

09 September 2018 7,604 1 View

Can anyone tell me how to remove the circled non dpecific staining? Is this due to the nature of the samples or the antibody?

Hi all, I have been observing these non specific staining whenever I perform Western blotting. I used Fast Blocking solution from Pierce, but to no avail. Tried lowering primary Ab, but my band...

05 May 2018 2,486 9 View

How to dissolve acidic peptide?

Hi all, I know there have been questions and answers on how to dissolve an acidic peptide. However, my questions will be: a) Can I dissolve my peptide using a basic solvent such as NaOH instead of...

09 September 2017 6,596 6 View

Cross reactivity problem in western blot?

Hi all, My western blot profile always show 2 clear bands at 110kDa and 75 kDa, no matter which secondary antibody I am using, be it rabbit and mouse secondary antibody. This problem will only...

04 April 2016 3,117 4 View