Literature on snowflake aggregation?

I am looking for literature suggestions on the topic of snow aggregation. My main interest is on the aggregation process itself (rather than papers describing aggregate snowflakes characteristics)...

19 February 2021 2,197 3 View

ASTM or ISO standard test for duplex coatings?

Dear all, I would like to conduct scratch and linear reciprocating tribo-test on my magensium alloy, which has a plasma electrolytic oxide (PEO) layer of about 20 um and 10 - 20 um of Parylene...

25 January 2021 9,709 2 View

Does anyone know about a good article/book about the correlation between the water crisis and the macroeconomic decisions?

I'm writing a thesis about the macroeconomic issues to face during a water crisis

25 December 2020 6,321 1 View

Any data or studies available on wall thermal insulation with glass bottles?

I am searching for data and studies about thermal performances of walls insulated using recycled unbroken glass bottles, to be used as part of the structure itself, or as filling material of a...

29 November 2020 7,792 2 View

EDX sample preparation of a coating/metal cross section?

The sample is piece a magnesium alloy (dimension: length - 20mm, width - 9.7mm, thickness - 3.5mm), which is coated with epoxy and silicone coating on each side of the surface. The coatings do not...

05 January 2020 3,375 3 View

How to modify a plasmid DNA with a thiol group or any functional groups for coupling?

I am trying to conjugate a plasmid DNA with an -SH activated PEG. The thiol group modification is possible for linear single strand DNA. I wonder if it possible to introduce in a plasmid DNA, too.

03 August 2019 135 1 View

Cloudy debris in the middle of a flask??

Hi everybody, I've started to grow up bacteria in liquid medium in order to set up a growth curve but everytime I try to start it there is this cloud in the middle of the flask. The bacteria...

26 March 2018 4,656 2 View

What are the basics to maintain a micropipette in good working conditions?

26 November 2014 9,393 4 View

Does anybody know the difference between GISTIC 2.0 and ABSOLUTE algorithms, if there is any?

While reading the literature,it is not very clear to me the rationale of using one or the another.

22 June 2014 4,609 1 View

Primary myoblast isolation and purification protocol?

Hi everyone, I working on a protocol using mouse gastrocnemius and TA muscle. So far I can see myoblasts and myocytes after about a week. However, after I try to differentiate using 5%HS my...

31 December 2019 881 0 View

Does the Horse serum must heat inactivated for the diffrentition of C2C12 myoblast cells ?

Hello, Please can you help me, I need to develop a C2C12 model in my laboratory and I would like to know if I should heat inactivated horse serum. Thank you for your answers! Have a beautiful...

09 October 2019 7,113 3 View

Does the Horse serum must heat inactivated for the diffrentition of C2C12 myoblast cells ?

Hello, Please can you help me, I need to develop a C2C12 model in my laboratory and I would like to know if I should heat inactivated horse serum. Thank you for your answers! Have a beautiful...

02 October 2019 3,097 3 View

What type of contamination is this?

This was observed in a chicken myoblast cell culture. In my media: Primocin until it ran out then I started using the Sigma's Antibiotic Antimycotic Solution. But the contamination was present...

07 August 2019 7,240 4 View

Does anyone have any experience in generating C2C12 and ST2 stable cell lines using lentiviral vectors?

I am trying to stably express either a WT or a mutant form of my protein of interest under the control of doxycycline (using pTRIPZ vector) in C2C12 and ST2 cells to study it's role in bone...

04 May 2019 1,131 2 View

Does FBS affect the alkaline phosphatase (ALP) assays and giving background readings?

I'm doing ALP assays on C2C12 myoblast as an osteogenic marker on the change of the differentiation pathway. But my negative control (C2C12 without osteogenic factors) gave me high values of ALP,...

03 April 2019 8,124 4 View

Are my C2C12s looking healthy?

I have been working for C2C12 mouse myoblast. Can anyone tell me if my cells are looking healthy? Are the ones pointed by red arrows looking normal? Should I be worrying?

03 April 2019 4,073 3 View

What is the maximum passage number we can go for H9c2 cells without losing its healthy morphology?

I am working with H9C2 cell line and I see a lot of differences in their morphology as they are giving more granules. And also they are forming clusters and not monolayer. The passage number is 25...

09 October 2018 3,663 1 View

Is H9c2 myoblast cell is suitable used to test the nanofiber-RGD scaffold for in vitro cell attachment and proliferation study?

I will synthesize nanofiber-RGD scaffold and need find a suitable cardiac cell to test the scaffold for cell attachment and proliferation. Currently, I find this cell line may suitable to use to...

08 September 2018 8,105 0 View

What is the optimal storage condition of Sodium fluoride (NaF)?

In lysis buffer, Sodium fluoride (NaF) act as a serine/threonine phosphatases inhibitor. I found different concentration as well as different storage condition depending on its application over...

24 June 2018 7,180 3 View