I am starting working with H9C2 cells and I am not sure how they were cultured before so I would like to reclone them to have a sure population of myoblasts.
I performed fluorescence stainings on brain formalin-fixed paraffin embedded samples (zebrafish). I stained TH+ neurons and counterstained with DAPI. In tissues coming from 2 specific animals,...
02 March 2023 9,072 6 View
Consider the special case of the Gagliardo--Nirenberg inequality in R^4 $$ \|u\|_p \le C_p \|\Delta u\|_2^{1-4/p} \|u\|_4^{4/p} $$ with $p \ge 4$. What is the value of the best constant $C_p$?
13 October 2021 1,142 5 View
Most portable medical O2 concentrators use pressure swing absorption to separate nitrogen from the air. Two sieve beds are filled with zeolite (13X is the most common), which absorbs Nitrogen at...
27 June 2021 2,956 0 View
Hi everybody, does anyone know a good reference for FETI in nonlinear static analysis? There is a lot of literature, but it's usually too advanced and I'd like to find just basic material...
12 March 2021 9,581 4 View
I am trying to find relevant literature about the possible magnetic attraction forces between snowflakes. Is it negligible or significant enough to trigger collision?
06 March 2021 1,418 11 View
I am looking for literature suggestions on the topic of snow aggregation. My main interest is on the aggregation process itself (rather than papers describing aggregate snowflakes characteristics)...
19 February 2021 2,467 3 View
Dear all, I would like to conduct scratch and linear reciprocating tribo-test on my magensium alloy, which has a plasma electrolytic oxide (PEO) layer of about 20 um and 10 - 20 um of Parylene...
25 January 2021 9,936 2 View
I'm writing a thesis about the macroeconomic issues to face during a water crisis
25 December 2020 6,509 1 View
I am searching for data and studies about thermal performances of walls insulated using recycled unbroken glass bottles, to be used as part of the structure itself, or as filling material of a...
29 November 2020 7,988 2 View
Hello everyone, i have a dataset of 64 ch EEG( i am fluent in the use of matlab, Fieldtrip, EEGlab and Brainstorm) I can easily obtain connectivity matrixes 64x64 from my EEG, i was willing to...
28 June 2020 8,466 0 View
I am designing research related to atherosclerosis in vitro, but unfortunately the cell lines currently available in our laboratory are only 3T3-L1 and H9C2, Does anyone can suggest me what...
17 July 2024 6,373 1 View
I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...
04 July 2024 4,732 1 View
How can isolate C2C12 myotube efficiently from C2C12 myoblast?
18 April 2024 6,123 0 View
i have been trying to differentiate primary human myoblasts into myotubes. i have early pssage (p2) and old pssage (p14). Hoth with same outocmes. the myoblasts have been growing in 20% fcs,...
27 February 2024 5,393 1 View
Hi, I'm just enquiring what seeding density people use for LHCN-M2 myoblasts and myotubes? Also how long do they leave RNAiMAX in the media for transfection before replacing with fresh media?
19 February 2024 2,624 0 View
Hello everyone, We needed cardiac cells for a project and obtained H9C2 rat cardiovascular myoblast cells from another laboratory. The cells are at passage 15 and unfortunately have...
07 October 2023 7,589 1 View
hi ... how i can make myotube formation from myoblast , without growth factors i used horse serum , and DMEM high glucose
03 September 2023 4,714 0 View
Someone said that transient Ca2+ depletion from the endoplasmic reticulum is critical for skeletal myoblast differentiation. I found that one gene deficiency in myoblast leads to precocious...
28 August 2023 2,916 0 View
The slow growth of H9C2 cells and the formation of cell clumps after centrifugation and resuspension might be caused by what factors?
20 August 2023 114 0 View
I need a method of making the scaffold in detail, as well as the method of culturing the cells, the appropriate culture media, and the additions, if any, to the medium
28 June 2023 7,403 0 View