How can isolate C2C12 myotube efficiently from C2C12 myoblast?
Cuáles fueron las tendencias en investigaciones en arquitectura, urbanismo y patrimonio edificado en decadas del 2000 al 2020? Porque requiero conocer tesis de posgrado nivel maestría...
24 July 2024 5,494 1 View
Hi, I have a question regarding western blot. I performed total protein statin after trasfering membrane. The result was totally fine before (Figure 1). However, there were strange bubble-like...
30 May 2024 8,045 3 View
Dear all researchers, I would appreciate if anyone here can assist me to find a survey instrument related to customer churn behaviors from previous study? I couldn't find it. Thanks.
19 May 2024 1,184 0 View
When refining with HighScore Plus, inserting the CIF file for gamma-Ca2SiO4 and then refining it will show "unusual space group setting for phase: gamma-Ca2SiO4". This occurs for all gamma-Ca2SiO4...
19 May 2024 10,033 1 View
The preprint papers on ResearchGate are not accessible/read by AI models such as ChatGPT and others. The papers are in image format but not PDF format. So the preprint papers on ResearchGate are...
17 May 2024 5,304 3 View
As the title described, it is common that a target gene sequence contains motif that acts as the binding site of transcription factor rather then the upstream regulatory region. Does this affect...
13 May 2024 1,099 3 View
As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...
29 April 2024 3,227 2 View
1. When using the input shaper, it is necessary to design the natural frequency and damping ratio. How to solve this natural frequency and damping ratio for the time-varying robot dynamics...
10 April 2024 378 0 View
It is my first time using SMARTpls and I stumbled upon a few issues that my books/readings do not seem to answer. The below are the issues. Thank you in advance for the guidance and advice. The...
29 March 2024 4,593 0 View
In the actual scenario of federated learning, the problem of heterogeneity is an inevitable challenge, so what can we do to alleviate the challenges caused by these heterogeneities?
19 March 2024 7,047 2 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View
What is the thermal expansion coefficient of human muscle?
27 July 2024 7,190 0 View
I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!
24 July 2024 3,460 1 View
I founde some textbooks stating that typical skeletal muscles can shorten up to 70% (optimally up to 30%) during contraction. However, I would like to look at muscles per region. This might seem...
21 July 2024 399 3 View
Electromyography waveforms reflect the electrical activity of a muscle. But does it reveal muscle spasms (transient) or cramps (prolonged spasm)? If so, what does it look like?
20 July 2024 5,951 1 View
I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...
04 July 2024 4,732 1 View
I'm having a bubbling error while splicing 100/350 um optical fiber (core/cladding) on the Fujikura FSM100P+. I have tried some ways such as changing Prefuse power and Prefuse time but to no...
03 July 2024 7,463 6 View
I am trying to visualize Nemaline Rods in Skeletal Muscle fiber cross sections. I have read about 10 different gomori protocols, and the protocol seems very straightforward. Every paper I read on...
27 June 2024 5,423 2 View
I'm not an electrical engineer or anything related to that subject, so my apologies if I understood something incorrectly. Well, I read somewhere that the slower the railgun is, the higher its...
23 June 2024 1,082 0 View
Hello, do you know what the phospholipids of the membrane of rat smooth muscle aortas are composed of? outer membrane-inner membrane
19 June 2024 221 0 View
