I am tyring identify if my treated cells are dying through apoptosis or necrosis using flow. Can anyone suggest a good Annexin V/PI flow cytometry protocol? Ideally one that you have used and had some joy with
Hi Nial,
I am using Annexin V FITC kit from invitrogen....here is the protocol they provoded..
http://probes.invitrogen.com/media/pis/mp13242.pdf
I am using Annexin V FITC kit from BD. Please find theattached protocol...
Best of luck for future experiments...
use kit for Annexin V FITC
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