Dear members of the community,
I am building a 15 % PCL scaffolds for cell culture dissolving the PCL with different solvents. I would like to know which one adsorbs more protein. That is why, I am conducting a protein adsorption assay. Nevertheless, the results does not show repeatability as sometimes I obtain high values and sometimes low values ( for an equally build scaffold). Does someone have any tip to understand why this is happenning?
Thank you in advance,
Enric
Dear Enric Casanova, the uniformity ans solubility of PCL have strong influence on adsorption. Please check the following documents. My Regards
https://www.researchgate.net/publication/26829793_Role_of_Nanofibrous_PolyCaprolactone_Scaffolds_in_Human_Mesenchymal_Stem_Cell_Attachment_and_Spreading_for_In_Vitro_Bone_Tissue_Engineering-Response_to_Osteogenic_Regulators
https://www.researchgate.net/publication/318112927_Protein_adsorption_and_cell_behaviors_on_polycaprolactone_film_The_effect_of_surface_topography
https://doi.org/10.1080/00222348.2016.1179245
Dear Enric Casanova, the uniformity ans solubility of PCL have strong influence on adsorption. Please check the following documents. My Regards
https://www.researchgate.net/publication/26829793_Role_of_Nanofibrous_PolyCaprolactone_Scaffolds_in_Human_Mesenchymal_Stem_Cell_Attachment_and_Spreading_for_In_Vitro_Bone_Tissue_Engineering-Response_to_Osteogenic_Regulators
https://www.researchgate.net/publication/318112927_Protein_adsorption_and_cell_behaviors_on_polycaprolactone_film_The_effect_of_surface_topography
https://doi.org/10.1080/00222348.2016.1179245
Hi;
The surface morphology of the PCL film has a profound effect on protein grafting. Indeed, the morphology of the film strongly depends on process variables such as the solubility parameter of the solvent, the evaporation rate of the solvent and the nature of the surface in contact with the polymer film during casting.
With my best regards
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