How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View

Does someone have accomplished electrospinning with 15 % PCL using chloroform or dicloromehtane as a solvent?

Dear community, I am trying to conduct electrospinning with 15% of PCL and I would like to do it with chloroform or dicloromethene. When I try to do it I do not manage to form a Taylor Cone that...

06 August 2020 3,823 6 View

No title

I uploaded a collaboration on my network but the 8 contributors that appear are wrong. The collaborator who has worked with me does not belong to the network. It would be necessary to erase the 8...

07 August 2018 5,129 0 View

Which are the differences in contact solution Abaqus 6.10 vs 6.14?

Hello all, Some time ago I solved a transient thermo-mechanical problem using Abaqus 6.10, and I was quite satisfied with the observed results (frictional dissipation energy and power loss due to...

28 December 2017 990 4 View

Which are the differences in contact solution Abaqus 6.10 vs 6.14?

Hello all, Some time ago I solved a transient thermo-mechanical problem using Abaqus 6.10, and I was quite satisfied with the observed results (frictional dissipation energy and power loss due to...

11 December 2017 8,231 5 View

Which surface markers do you use to assess differentiation by PMAin K562?

Which surface markers do you use to assess differentiation by PMA (TPA) in K562 by flow cytometry? I have been checking some papers but I am still not sure which are the appropiate cell surface...

02 August 2017 1,092 2 View

Does anyone have BVH files cutter software or procedure?

25 April 2015 6,178 2 View

About the HSCL-20 (in Spanish).

I look forward to using the HSCL-20 questionnaire to monitor the evolution of depressive symptoms in a research project. This scale is widely used in US clinical trials on disease management...

13 March 2013 1,705 1 View

Protein adsorption, large variation

Dear members of the community, I am building a 15 % PCL scaffolds for cell culture dissolving the PCL with different solvents. I would like to know which one adsorbs more protein. That is why, I...

01 January 1970 9,338 3 View

How do you visualize the future of remote learning after the pandemic?

In the context of the COVID-19 pandemic, the boundaries and conflicts between face-to-face and distance education have been erased. In my concept this time has led to a shift, toward hybrid...

01 January 1970 3,241 36 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Not able to charge-discharge Li-ion coin cell at higher C rate ?

I am working on cathode material. I have made different Li-ion coin cell against Li metal using commercial available cathode materials such as LCO,LMO, LFP and NMC and also with the lab...

18 February 2021 1,450 3 View